Super Mario Bros 2

In this North American sequel to the classic Super Mario Bros., Mario, Luigi, Toad, and Peach venture across twenty levels of the magical kingdom of Sub-Con to rescue its kidnapped inhabitants and defeat the evil usurper king Wart. SMB2 (originally an unrelated Famicom Disk System game titled Doki Doki Panic in Japan but retooled as a Mario game for its US release) was the first American game to establish that Luigi has a higher jump than his brother and that Peach can float using her dress, both of which would become defining characteristics in later titles. It also introduced a whole new cast of enemies to the Mario universe, including now-regulars like Shyguys, Bob-ombs, and Birdo.